Sustainable Mining and Metallurgy

This work explored the feasibility of dewatered swine manure-derived biochar (DSMB) as an additive to facilitate anaerobic digestion (AD) of swine wastewater for energy recovery and antibiotic resistance genes (ARG) attenuation enhancements. With 20g/L DSMB assistance, the methanogenic lag time of swine wastewater was shortened by 2~3 days, and the maximum methane production was increased by 18.29%~23.58%.The addition of DSMB could significantly accelerate the degradation rate of soluble COD and VFA,and the performance of DSMB prepared at 300℃ is better than that prepared at 500℃ and 700℃. The results of microbial community analysis show that the relative abundance of Thermotogae in DSMB prepared at 300℃ is more then three times that in the control group, which accelerates the accurrence of hydrolysis and acidification stages. The INT-ETS activity and EEC analysis show that DSMB prepared at 300℃ acts as redox active medium to accelerate methane production through potential direct interspecies electron transfer (DIET) pathway.